Background: The aim of the study was to investigate the influence of local anesthetics on biofilm-forming properties of clinical isolates S. aureus and their biofilm-formation on venous catheters made of different polymers.
Materials and methods: The study was based on investigating 62 clinical strains of S. aureus isolated from surgical patients. Biofilm-forming properties of clinical strains S. aureus were assessed by using the Christensen’s spectrophotometric method. S. aureus biofilms were cultivated on polyurethane and teflon catheters by immersion in broth medium inoculated by suspension of bacterial culture. Also biofilm-formation on the surfaces of various catheters was observed by microscopic examination after their removal from the culture medium, washing with sterile saline and staining with crystal violet. In the study we used bupivacaine 0.125 %, 0.25 % and lidocaine 0.5 %, 1.0 %.
Results: The findings obtained demonstrated that clinical isolates S. aureus have high biofilm-forming potential, their optical density unit (ODU) was 0.244 ± 0.06. Bupivacaine in concentration 0.125 % contributed to a decrease in the optical density of the biofilms of the investigated species in 0.7 times (p = 0.001), compared with their indices without the presence of the anesthetics. There was a decrease in the film-forming properties of investigated isolates in the presence of lidocaine in concentrations 0.5 % and 1.0 % in a 1.13 time and 1.15 times respectively. The ODU values of the film forms obtained by us correlate with the results of microscopic examination.
Conclusion: 0.5 % and 1.0 % lidocaine and 0.25 % bupivacaine significantly inhibit the biofilm-production of S. aureus clinical isolates and formation of biofilms on the surfaces of catheters from different polymers.
Key words: biofilm, local anesthetics, polymers, resistance, venous catheters.
Lek Obz, 2020, 70 (1): 21-24
Článok vyšiel v Anglickom jazyku
Dmytro Dmytriiev 1, Oleksandr Nazarchuk 1, Mariia Faustova 2, Yuliana Babina 1, Kostyantin Dmytriiev 1, Oleksandr Dobrovanov 3,4, Karol Kralinský 5,6
1 National Pirogov Memorial Medical University, Vinnytsya, Ukraine
2 Ukrainian Medical Stomatological Academy, Poltava, Ukraine.
3 A. Getlik Clinic for Children and Adolescents, Slovak Medical University and University Hospital of Medicine, Bratislava, Slovakia, head doc. MUDr. Katarina Furkova, CSc.
4 St. Elizabeth University of Health and Social sciences, Bratislava, Slovakia
5 2nd Children‘s Clinic of Slovak Medical University, Children Faculty Hospital with Polyclinic, Banska Bystrica, Slovakia, head prof. MUDr. Karol Kralinsky, PhD.
6 Faculty of Health Care of Slovak Medical University in Bratislava, based in Banska Bystrica, Slovakia
Dmytriiev D., Nazarchuk O., Faustova M., Babina Y., Dmytriiev K., Dobrovanov O., Kralinský K.: Local anesthetics contributing the struggling biofilm-forming activity of S. aureus colonizing surfaces of venous catheters. Multicentrer clinical trial. Lek Obz, 2020, 70 (1): 21-24
Dmytriiev D., Nazarchuk O., Faustova M., Babina Y., Dmytriiev K., Dobrovanov O., Kralinský K.: Vplyv lokálnych anestetík na tvorbu mikrobiálneho biofilmu S. aureus na povrchu venóznych katétrov. Multicentrická klinická štúdia Lek Obz, 2020, 70, č. 1, s. 21-24